REACTIONS OF TRYPSIN AND CHYMOTRYPSIN WITH HEPARIN, TRYPSIN INHIBITOR, AND HEXYLRESORCINOL
نویسندگان
چکیده
منابع مشابه
Reactions of Trypsin and Chymotrypsin with Heparin, Trypsin Inhibitor, and Hexylresorcinol
il study of enzyme inhibitors designed to obtain information on the possible physiological significance of blood antiprotease has produced a number of simple fundamental reactions which have hitherto not been reported. It is the purpose of this paper to record these reactions for the possible benefit of others who may wish to investigate them further. Materials-The t,rypsin, chymotrypsin, and t...
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The heats of reaction of trypsin with soybean (Kunitz), ovomucoid, and lima bean inhibitors have been measured at pH 5.0 and 10’ and 25”. All the reactions were endothermic, with AH ranging from 8.6 Cal per mole for the lima bean inhibitor to 15.3 Cal per mole for the soybean inhibitor. Equilibrium constants for the association were calculated from the dependence of residual tryptic activity up...
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1. A trypsin and chymotrypsin inhibitor was isolated by extraction of chick-pea meal at pH8.3, followed by (NH4)2SO4 precipitation and successive column chromatography on CM-cellulose and calcium phosphate (hydroxyapatite). 2. The inhibitor was pure by polyacrylamide-gel and cellulose acetate electrophoresis and by isoelectric focusing in polyacrylamide gels. 3. The inhibitor had a molecular we...
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2.1. Materials One approach to circumvent this difficulty is to introduce an indirect signal by coupling an indicator reaction to the reaction of interest. Examples are monitoring the enzymatic activity of free enzyme [2] or following the displacement of proflavin from the enzyme when the inhibitor is bound [3]. With all these indirect measurements careful studies as to possible side reactions ...
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Methods are described for the isolation and crystallization of trypsinogen, trypsin, a substance which inhibits trypsin, and an inhibitor-trypsin compound. Analyses and some of the properties of these compounds are given.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1944
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)51123-6